Development of a New Screen-Printed Transducer for the Electrochemical Detection of Thiram

A new transducer based on a screen-printed carbon electrode has been developed for the quantification of thiram. Detection this fungicide is performance two enzymes: (1) aldehyde dehydrogenase catalyzes oxidation using NAD+ as cofactor and simultaneously, (2) diaphorase reoxidizes NADH formed in first enzymatic process due to presence hexacyanoferrate(III) which reduced hexacyanoferrate(II). Taking into account that inhibited by thiram, current decreases with pesticide concentration thiram can be electrochemically quantified below legal limits. The proposed work involves modification WE co-factors (NAD+ hexacyanoferrate(III)) required system. device employed allows detection 0.09 ppm limits (Maximum Residue Limits 0.1–10 ppm).